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phusion high fidelity pcr master mix  (New England Biolabs)


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    New England Biolabs phusion high fidelity pcr master mix
    Phusion High Fidelity Pcr Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6456 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hf+buffer/Phusion+High-Fidelity+PCR+Master+Mix+with+HF+Buffer/10__1016_slash_j__fochx__2026__104605-70-5-10
    Average 99 stars, based on 6456 article reviews
    phusion high fidelity pcr master mix - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Knock-In:

    Article Title: Generating cisgenic sexing strains in insect pests.
    Article Snippet: .. The knock-in site was amplified using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) with genome-specific 1167A_F and 1167A_R primers (Supplementary Table 2) designed in Geneious Prime 2023.1.2. .. To confirm the initial integration, the PCR products were purified via Monarch® PCR & DNA Cleanup Kit (New England Biolabs®) and analysed via Oxford Nanopore sequencing (Full Circle Labs).

    Amplification:

    Article Title: Generating cisgenic sexing strains in insect pests.
    Article Snippet: .. The knock-in site was amplified using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) with genome-specific 1167A_F and 1167A_R primers (Supplementary Table 2) designed in Geneious Prime 2023.1.2. .. To confirm the initial integration, the PCR products were purified via Monarch® PCR & DNA Cleanup Kit (New England Biolabs®) and analysed via Oxford Nanopore sequencing (Full Circle Labs).

    Article Title: Generating cisgenic sexing strains in insect pests.
    Article Snippet: RNA was extracted via an adapted TRIzol® (Ambion)chloroform-based protocol (53). cDNA was synthesised from total RNA using Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase (ThermoFisher) according to the instructions provided by the manufacturer. .. Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) was used for PCR amplification from gDNA and cDNA templates using the 1167_F_V1 splicing and 1167A_R primer pair (Supplementary Table 2). .. Bands amplified from cDNA of the IMPERIAL strain were purified using a Monarch® DNA Gel Extraction Kit (New England Biolabs®) and Sanger sequenced (Genewiz Inc.).

    Polymerase Chain Reaction:

    Article Title: Generating cisgenic sexing strains in insect pests.
    Article Snippet: .. The knock-in site was amplified using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) with genome-specific 1167A_F and 1167A_R primers (Supplementary Table 2) designed in Geneious Prime 2023.1.2. .. To confirm the initial integration, the PCR products were purified via Monarch® PCR & DNA Cleanup Kit (New England Biolabs®) and analysed via Oxford Nanopore sequencing (Full Circle Labs).

    Article Title: Mapping the GCK–GKRP interaction landscape using deep mutational scanning by reverse two-hybrid screening
    Article Snippet: .. The plasmid DNA from each regional replicate was adjusted to equal concentrations, and was then used as template in a PCR to amplify each tile, with each reaction consisting of: 20 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 1 μL 10 μM forward primer, 1 μL 10 μM reverse primer, 18 μL plasmid library template. ..

    Article Title: Discovery of novel murine PML isoforms
    Article Snippet: .. PCR was performed with Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs, Cat. No. M0531) using 1 μl of cDNA template per 25-μl reaction volume under the following conditions: an initial denaturation at 98°C for 30 s, 35 cycles of denaturation at 98°C for 10 s, annealing at 57–67°C for 30 s, and extension at 72°C for 1 min; and a final extension at 72°C for 10 min. ..

    Article Title: Mapping the GCK–GKRP interaction landscape using deep mutational scanning by reverse two-hybrid screening
    Article Snippet: .. Each reaction consisted of the following: 20 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 2 μL 10 μM i5 indexing adapter, 2 μL 10 μM i7 indexing adapter, 1 μL 1:10 diluted PCR product, 15 μL nuclease-free water. .. Illumina index adaptors were added using the following PCR program: 98°C 30 sec, 7 cycles of 98 °C 15 sec, 65 °C 30 sec, 72 °C 120 sec, followed by 72 °C 7 min and hold at 4 °C.

    Article Title: A Functional Genetic Atlas of Parkin Resolves Variants of Uncertain Significance and Predicts Parkinson’s Disease Age at Onset
    Article Snippet: .. Each reaction contained 12.5 μL of Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs, M0531S), 500 ng of genomic DNA, 10 μM of a reverse primer carrying the Illumina P5 sequence (VA04), and 10 μM of an indexed forward primer incorporating the Illumina P7 sequence (VA05) that anneals upstream of the barcode. ..

    Article Title: Generating cisgenic sexing strains in insect pests.
    Article Snippet: RNA was extracted via an adapted TRIzol® (Ambion)chloroform-based protocol (53). cDNA was synthesised from total RNA using Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase (ThermoFisher) according to the instructions provided by the manufacturer. .. Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs®) was used for PCR amplification from gDNA and cDNA templates using the 1167_F_V1 splicing and 1167A_R primer pair (Supplementary Table 2). .. Bands amplified from cDNA of the IMPERIAL strain were purified using a Monarch® DNA Gel Extraction Kit (New England Biolabs®) and Sanger sequenced (Genewiz Inc.).

    Article Title: Devices and methods for mitochondria replacement and for generating cellular therapeutics
    Article Snippet: Total DNA was extracted from 1×106 cells using the Qiagen DNasy Blood and Tissue kit. .. PCR was performed using Phusion high-fidelity PCR master mix with HF buffer (NEB, Cat. #M0531S) and the following primers: forward-TTCCAAGGACAAATCAGAGAAAAAGT (SEQ ID NO: 5), reverse-AGCCCGTCTAAACATTTTCAGTGTA (SEQ ID NO: 6). .. PCR was run on an Eppendorf vapo.protect thermal cycler at 1) 98° C. for 2 min, 2) 30 cycles −98° C. for 15 s, 58° C. for 30 s, 72° C. for 30 s, and 3) 72° C. for 5 min. PCR products were run on a 0.8-1% agarose TAE gel, extracted with the QIAGEN QIAQuick Gel Extraction kit (Qiagen, Cat. #28704), and Sanger sequenced using the same PCR primers.

    Article Title: Discovery of novel murine PML isoforms
    Article Snippet: .. PCR was performed with Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs, Cat. No. M0531) using 2 μl of cDNA template per 50-μl reaction volume. ..

    Plasmid Preparation:

    Article Title: Mapping the GCK–GKRP interaction landscape using deep mutational scanning by reverse two-hybrid screening
    Article Snippet: .. The plasmid DNA from each regional replicate was adjusted to equal concentrations, and was then used as template in a PCR to amplify each tile, with each reaction consisting of: 20 μL Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB), 1 μL 10 μM forward primer, 1 μL 10 μM reverse primer, 18 μL plasmid library template. ..

    Sequencing:

    Article Title: A Functional Genetic Atlas of Parkin Resolves Variants of Uncertain Significance and Predicts Parkinson’s Disease Age at Onset
    Article Snippet: .. Each reaction contained 12.5 μL of Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs, M0531S), 500 ng of genomic DNA, 10 μM of a reverse primer carrying the Illumina P5 sequence (VA04), and 10 μM of an indexed forward primer incorporating the Illumina P7 sequence (VA05) that anneals upstream of the barcode. ..



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